diff --git a/homeassistant/components/climate/__init__.py b/homeassistant/components/climate/__init__.py
index 7213a2ebca0..1aa082f8c6c 100644
--- a/homeassistant/components/climate/__init__.py
+++ b/homeassistant/components/climate/__init__.py
@@ -1008,11 +1008,22 @@ async def async_service_temperature_set(
         )
 
     hass = entity.hass
-    kwargs = {}
+    kwargs: dict[str, Any] = {}
     min_temp = entity.min_temp
     max_temp = entity.max_temp
     temp_unit = entity.temperature_unit
 
+    if (
+        (target_low_temp := service_call.data.get(ATTR_TARGET_TEMP_LOW))
+        and (target_high_temp := service_call.data.get(ATTR_TARGET_TEMP_HIGH))
+        and target_low_temp > target_high_temp
+    ):
+        # Ensure target_low_temp is not higher than target_high_temp.
+        raise ServiceValidationError(
+            translation_domain=DOMAIN,
+            translation_key="low_temp_higher_than_high_temp",
+        )
+
     for value, temp in service_call.data.items():
         if value in CONVERTIBLE_ATTRIBUTE:
             kwargs[value] = check_temp = TemperatureConverter.convert(
diff --git a/homeassistant/components/climate/strings.json b/homeassistant/components/climate/strings.json
index 3ff8d325da5..fc0bdaf0d72 100644
--- a/homeassistant/components/climate/strings.json
+++ b/homeassistant/components/climate/strings.json
@@ -270,6 +270,9 @@
     "temp_out_of_range": {
       "message": "Provided temperature {check_temp} is not valid. Accepted range is {min_temp} to {max_temp}."
     },
+    "low_temp_higher_than_high_temp": {
+      "message": "Target temperature low can not be higher than Target temperature high."
+    },
     "humidity_out_of_range": {
       "message": "Provided humidity {humidity} is not valid. Accepted range is {min_humidity} to {max_humidity}."
     }
diff --git a/tests/components/climate/test_init.py b/tests/components/climate/test_init.py
index 1c9144b40f7..2b09c2801df 100644
--- a/tests/components/climate/test_init.py
+++ b/tests/components/climate/test_init.py
@@ -1224,3 +1224,66 @@ async def test_temperature_validation(
     state = hass.states.get("climate.test")
     assert state.attributes.get(ATTR_TARGET_TEMP_LOW) == 10
     assert state.attributes.get(ATTR_TARGET_TEMP_HIGH) == 25
+
+
+async def test_target_temp_high_higher_than_low(
+    hass: HomeAssistant, register_test_integration: MockConfigEntry
+) -> None:
+    """Test that target high is higher than target low."""
+
+    class MockClimateEntityTemp(MockClimateEntity):
+        """Mock climate class with mocked aux heater."""
+
+        _attr_supported_features = (
+            ClimateEntityFeature.TARGET_TEMPERATURE
+            | ClimateEntityFeature.TARGET_TEMPERATURE_RANGE
+        )
+        _attr_current_temperature = 15
+        _attr_target_temperature = 15
+        _attr_target_temperature_high = 18
+        _attr_target_temperature_low = 10
+        _attr_target_temperature_step = PRECISION_WHOLE
+
+        def set_temperature(self, **kwargs: Any) -> None:
+            """Set new target temperature."""
+            if ATTR_TEMPERATURE in kwargs:
+                self._attr_target_temperature = kwargs[ATTR_TEMPERATURE]
+            if ATTR_TARGET_TEMP_HIGH in kwargs:
+                self._attr_target_temperature_high = kwargs[ATTR_TARGET_TEMP_HIGH]
+                self._attr_target_temperature_low = kwargs[ATTR_TARGET_TEMP_LOW]
+
+    test_climate = MockClimateEntityTemp(
+        name="Test",
+        unique_id="unique_climate_test",
+    )
+
+    setup_test_component_platform(
+        hass, DOMAIN, entities=[test_climate], from_config_entry=True
+    )
+    await hass.config_entries.async_setup(register_test_integration.entry_id)
+    await hass.async_block_till_done()
+
+    state = hass.states.get("climate.test")
+    assert state.attributes.get(ATTR_CURRENT_TEMPERATURE) == 15
+    assert state.attributes.get(ATTR_MIN_TEMP) == 7
+    assert state.attributes.get(ATTR_MAX_TEMP) == 35
+
+    with pytest.raises(
+        ServiceValidationError,
+        match="Target temperature low can not be higher than Target temperature high",
+    ) as exc:
+        await hass.services.async_call(
+            DOMAIN,
+            SERVICE_SET_TEMPERATURE,
+            {
+                "entity_id": "climate.test",
+                ATTR_TARGET_TEMP_HIGH: "15",
+                ATTR_TARGET_TEMP_LOW: "20",
+            },
+            blocking=True,
+        )
+    assert (
+        str(exc.value)
+        == "Target temperature low can not be higher than Target temperature high"
+    )
+    assert exc.value.translation_key == "low_temp_higher_than_high_temp"