diff --git a/homeassistant/components/motioneye/media_source.py b/homeassistant/components/motioneye/media_source.py
index 85fe3985b93..20fc4359ab2 100644
--- a/homeassistant/components/motioneye/media_source.py
+++ b/homeassistant/components/motioneye/media_source.py
@@ -284,7 +284,13 @@ class MotionEyeMediaSource(MediaSource):
 
         sub_dirs: set[str] = set()
         parts = parsed_path.parts
-        for media in resp.get(KEY_MEDIA_LIST, []):
+        media_list = resp.get(KEY_MEDIA_LIST, [])
+
+        def get_media_sort_key(media: dict) -> str:
+            """Get media sort key."""
+            return media.get(KEY_PATH, "")
+
+        for media in sorted(media_list, key=get_media_sort_key):
             if (
                 KEY_PATH not in media
                 or KEY_MIME_TYPE not in media
diff --git a/tests/components/motioneye/test_media_source.py b/tests/components/motioneye/test_media_source.py
index 541db872b51..b4803c52a6d 100644
--- a/tests/components/motioneye/test_media_source.py
+++ b/tests/components/motioneye/test_media_source.py
@@ -250,6 +250,20 @@ async def test_async_browse_media_success(hass: HomeAssistant) -> None:
         "children_media_class": "video",
         "thumbnail": None,
         "children": [
+            {
+                "title": "00-02-27.mp4",
+                "media_class": "video",
+                "media_content_type": "video/mp4",
+                "media_content_id": (
+                    "media-source://motioneye"
+                    f"/74565ad414754616000674c87bdc876c#{device.id}#movies#"
+                    "/2021-04-25/00-02-27.mp4"
+                ),
+                "can_play": True,
+                "can_expand": False,
+                "thumbnail": "http://movie",
+                "children_media_class": None,
+            },
             {
                 "title": "00-26-22.mp4",
                 "media_class": "video",
@@ -278,20 +292,6 @@ async def test_async_browse_media_success(hass: HomeAssistant) -> None:
                 "thumbnail": "http://movie",
                 "children_media_class": None,
             },
-            {
-                "title": "00-02-27.mp4",
-                "media_class": "video",
-                "media_content_type": "video/mp4",
-                "media_content_id": (
-                    "media-source://motioneye"
-                    f"/74565ad414754616000674c87bdc876c#{device.id}#movies#"
-                    "/2021-04-25/00-02-27.mp4"
-                ),
-                "can_play": True,
-                "can_expand": False,
-                "thumbnail": "http://movie",
-                "children_media_class": None,
-            },
         ],
         "not_shown": 0,
     }