diff --git a/homeassistant/components/matrix/__init__.py b/homeassistant/components/matrix/__init__.py
index 8640aa4d074..5123436a397 100644
--- a/homeassistant/components/matrix/__init__.py
+++ b/homeassistant/components/matrix/__init__.py
@@ -475,7 +475,7 @@ class MatrixBot:
         file_stat = await aiofiles.os.stat(image_path)
 
         _LOGGER.debug("Uploading file from path, %s", image_path)
-        async with aiofiles.open(image_path, "r+b") as image_file:
+        async with aiofiles.open(image_path, "rb") as image_file:
             response, _ = await self._client.upload(
                 image_file,
                 content_type=mime_type,