diff --git a/homeassistant/components/intent_script/__init__.py b/homeassistant/components/intent_script/__init__.py
index 2ec898bfb0e..55c4947fe4a 100644
--- a/homeassistant/components/intent_script/__init__.py
+++ b/homeassistant/components/intent_script/__init__.py
@@ -5,9 +5,16 @@ import logging
 
 import voluptuous as vol
 
-from homeassistant.const import CONF_TYPE
-from homeassistant.core import HomeAssistant
-from homeassistant.helpers import config_validation as cv, intent, script, template
+from homeassistant.const import CONF_TYPE, SERVICE_RELOAD
+from homeassistant.core import HomeAssistant, ServiceCall
+from homeassistant.helpers import (
+    config_validation as cv,
+    intent,
+    script,
+    service,
+    template,
+)
+from homeassistant.helpers.reload import async_integration_yaml_config
 from homeassistant.helpers.typing import ConfigType
 
 _LOGGER = logging.getLogger(__name__)
@@ -55,10 +62,27 @@ CONFIG_SCHEMA = vol.Schema(
 )
 
 
-async def async_setup(hass: HomeAssistant, config: ConfigType) -> bool:
-    """Set up the intent script component."""
-    intents = config[DOMAIN]
+async def async_reload(hass: HomeAssistant, servie_call: ServiceCall) -> None:
+    """Handle start Intent Script service call."""
+    new_config = await async_integration_yaml_config(hass, DOMAIN)
+    existing_intents = hass.data[DOMAIN]
+
+    for intent_type in existing_intents:
+        intent.async_remove(hass, intent_type)
+
+    if not new_config or DOMAIN not in new_config:
+        hass.data[DOMAIN] = {}
+        return
+
+    new_intents = new_config[DOMAIN]
+
+    async_load_intents(hass, new_intents)
+
+
+def async_load_intents(hass: HomeAssistant, intents: dict):
+    """Load YAML intents into the intent system."""
     template.attach(hass, intents)
+    hass.data[DOMAIN] = intents
 
     for intent_type, conf in intents.items():
         if CONF_ACTION in conf:
@@ -67,6 +91,23 @@ async def async_setup(hass: HomeAssistant, config: ConfigType) -> bool:
             )
         intent.async_register(hass, ScriptIntentHandler(intent_type, conf))
 
+
+async def async_setup(hass: HomeAssistant, config: ConfigType) -> bool:
+    """Set up the intent script component."""
+    intents = config[DOMAIN]
+
+    async_load_intents(hass, intents)
+
+    async def _handle_reload(servie_call: ServiceCall) -> None:
+        return await async_reload(hass, servie_call)
+
+    service.async_register_admin_service(
+        hass,
+        DOMAIN,
+        SERVICE_RELOAD,
+        _handle_reload,
+    )
+
     return True
 
 
diff --git a/homeassistant/components/intent_script/services.yaml b/homeassistant/components/intent_script/services.yaml
new file mode 100644
index 00000000000..bb981dbc69c
--- /dev/null
+++ b/homeassistant/components/intent_script/services.yaml
@@ -0,0 +1,3 @@
+reload:
+  name: Reload
+  description: Reload the intent_script configuration.
diff --git a/homeassistant/helpers/intent.py b/homeassistant/helpers/intent.py
index 8b07c2adc9a..f2b29c0040b 100644
--- a/homeassistant/helpers/intent.py
+++ b/homeassistant/helpers/intent.py
@@ -57,6 +57,16 @@ def async_register(hass: HomeAssistant, handler: IntentHandler) -> None:
     intents[handler.intent_type] = handler
 
 
+@callback
+@bind_hass
+def async_remove(hass: HomeAssistant, intent_type: str) -> None:
+    """Remove an intent from Home Assistant."""
+    if (intents := hass.data.get(DATA_KEY)) is None:
+        return
+
+    intents.pop(intent_type, None)
+
+
 @bind_hass
 async def async_handle(
     hass: HomeAssistant,
diff --git a/tests/components/intent_script/fixtures/configuration.yaml b/tests/components/intent_script/fixtures/configuration.yaml
new file mode 100644
index 00000000000..93b4ebe5e26
--- /dev/null
+++ b/tests/components/intent_script/fixtures/configuration.yaml
@@ -0,0 +1,4 @@
+intent_script:
+  NewIntent2:
+    speech:
+      text: Hello World
diff --git a/tests/components/intent_script/fixtures/configuration_no_entry.yaml b/tests/components/intent_script/fixtures/configuration_no_entry.yaml
new file mode 100644
index 00000000000..e69de29bb2d
diff --git a/tests/components/intent_script/test_init.py b/tests/components/intent_script/test_init.py
index 044bbcaa7e7..a68b2a9be24 100644
--- a/tests/components/intent_script/test_init.py
+++ b/tests/components/intent_script/test_init.py
@@ -1,9 +1,14 @@
 """Test intent_script component."""
+from unittest.mock import patch
+
+from homeassistant import config as hass_config
 from homeassistant.bootstrap import async_setup_component
+from homeassistant.components.intent_script import DOMAIN
+from homeassistant.const import SERVICE_RELOAD
 from homeassistant.core import HomeAssistant
 from homeassistant.helpers import intent
 
-from tests.common import async_mock_service
+from tests.common import async_mock_service, get_fixture_path
 
 
 async def test_intent_script(hass: HomeAssistant) -> None:
@@ -134,3 +139,49 @@ async def test_intent_script_falsy_reprompt(hass: HomeAssistant) -> None:
 
     assert response.card["simple"]["title"] == "Hello Paulus"
     assert response.card["simple"]["content"] == "Content for Paulus"
+
+
+async def test_reload(hass: HomeAssistant) -> None:
+    """Verify we can reload intent config."""
+
+    config = {"intent_script": {"NewIntent1": {"speech": {"text": "HelloWorld123"}}}}
+
+    await async_setup_component(hass, "intent_script", config)
+    await hass.async_block_till_done()
+
+    intents = hass.data.get(intent.DATA_KEY)
+
+    assert len(intents) == 1
+    assert intents.get("NewIntent1")
+
+    yaml_path = get_fixture_path("configuration.yaml", "intent_script")
+
+    with patch.object(hass_config, "YAML_CONFIG_FILE", yaml_path):
+        await hass.services.async_call(
+            DOMAIN,
+            SERVICE_RELOAD,
+            {},
+            blocking=True,
+        )
+        await hass.async_block_till_done()
+
+    assert len(intents) == 1
+
+    assert intents.get("NewIntent1") is None
+    assert intents.get("NewIntent2")
+
+    yaml_path = get_fixture_path("configuration_no_entry.yaml", "intent_script")
+
+    with patch.object(hass_config, "YAML_CONFIG_FILE", yaml_path):
+        await hass.services.async_call(
+            DOMAIN,
+            SERVICE_RELOAD,
+            {},
+            blocking=True,
+        )
+        await hass.async_block_till_done()
+
+    # absence of intent_script from the configuration.yaml should delete all intents.
+    assert len(intents) == 0
+    assert intents.get("NewIntent1") is None
+    assert intents.get("NewIntent2") is None
diff --git a/tests/helpers/test_intent.py b/tests/helpers/test_intent.py
index edc6a281172..f3256e90b62 100644
--- a/tests/helpers/test_intent.py
+++ b/tests/helpers/test_intent.py
@@ -1,4 +1,6 @@
 """Tests for the intent helpers."""
+from unittest.mock import MagicMock, patch
+
 import pytest
 import voluptuous as vol
 
@@ -184,3 +186,63 @@ async def test_cant_turn_on_lock(hass: HomeAssistant) -> None:
 
     assert result.response.response_type == intent.IntentResponseType.ERROR
     assert result.response.error_code == intent.IntentResponseErrorCode.NO_INTENT_MATCH
+
+
+def test_async_register(hass: HomeAssistant) -> None:
+    """Test registering an intent and verifying it is stored correctly."""
+    handler = MagicMock()
+    handler.intent_type = "test_intent"
+
+    intent.async_register(hass, handler)
+
+    assert hass.data[intent.DATA_KEY]["test_intent"] == handler
+
+
+def test_async_register_overwrite(hass: HomeAssistant) -> None:
+    """Test registering multiple intents with the same type, ensuring the last one overwrites the previous one and a warning is emitted."""
+    handler1 = MagicMock()
+    handler1.intent_type = "test_intent"
+
+    handler2 = MagicMock()
+    handler2.intent_type = "test_intent"
+
+    with patch.object(intent._LOGGER, "warning") as mock_warning:
+        intent.async_register(hass, handler1)
+        intent.async_register(hass, handler2)
+
+        mock_warning.assert_called_once_with(
+            "Intent %s is being overwritten by %s", "test_intent", handler2
+        )
+
+    assert hass.data[intent.DATA_KEY]["test_intent"] == handler2
+
+
+def test_async_remove(hass: HomeAssistant) -> None:
+    """Test removing an intent and verifying it is no longer present in the Home Assistant data."""
+    handler = MagicMock()
+    handler.intent_type = "test_intent"
+
+    intent.async_register(hass, handler)
+    intent.async_remove(hass, "test_intent")
+
+    assert "test_intent" not in hass.data[intent.DATA_KEY]
+
+
+def test_async_remove_no_existing_entry(hass: HomeAssistant) -> None:
+    """Test the removal of a non-existing intent from Home Assistant's data."""
+    handler = MagicMock()
+    handler.intent_type = "test_intent"
+    intent.async_register(hass, handler)
+
+    intent.async_remove(hass, "test_intent2")
+
+    assert "test_intent2" not in hass.data[intent.DATA_KEY]
+
+
+def test_async_remove_no_existing(hass: HomeAssistant) -> None:
+    """Test the removal of an intent where no config exists."""
+
+    intent.async_remove(hass, "test_intent2")
+    # simply shouldn't cause an exception
+
+    assert intent.DATA_KEY not in hass.data