diff --git a/tests/components/backup/conftest.py b/tests/components/backup/conftest.py
index eb38399eb79..8c0e0ef63ac 100644
--- a/tests/components/backup/conftest.py
+++ b/tests/components/backup/conftest.py
@@ -61,24 +61,48 @@ def path_glob_fixture(hass: HomeAssistant) -> Generator[MagicMock]:
 
 
 CONFIG_DIR = {
-    "testing_config": [
+    "tests/testing_config": [
         Path("test.txt"),
         Path(".DS_Store"),
         Path(".storage"),
+        Path("another_subdir"),
         Path("backups"),
         Path("tmp_backups"),
+        Path("tts"),
         Path("home-assistant_v2.db"),
     ],
-    "backups": [
+    "/backups": [
         Path("backups/backup.tar"),
         Path("backups/not_backup"),
     ],
-    "tmp_backups": [
+    "/another_subdir": [
+        Path("another_subdir/backups"),
+        Path("another_subdir/tts"),
+    ],
+    "another_subdir/backups": [
+        Path("another_subdir/backups/backup.tar"),
+        Path("another_subdir/backups/not_backup"),
+    ],
+    "another_subdir/tts": [
+        Path("another_subdir/tts/voice.mp3"),
+    ],
+    "/tmp_backups": [  # noqa: S108
         Path("tmp_backups/forgotten_backup.tar"),
         Path("tmp_backups/not_backup"),
     ],
+    "/tts": [
+        Path("tts/voice.mp3"),
+    ],
+}
+CONFIG_DIR_DIRS = {
+    Path(".storage"),
+    Path("another_subdir"),
+    Path("another_subdir/backups"),
+    Path("another_subdir/tts"),
+    Path("backups"),
+    Path("tmp_backups"),
+    Path("tts"),
 }
-CONFIG_DIR_DIRS = {Path(".storage"), Path("backups"), Path("tmp_backups")}
 
 
 @pytest.fixture(name="create_backup")
@@ -105,7 +129,10 @@ def mock_backup_generation_fixture(
     """Mock backup generator."""
 
     with (
-        patch("pathlib.Path.iterdir", lambda x: CONFIG_DIR.get(x.name, [])),
+        patch(
+            "pathlib.Path.iterdir",
+            lambda x: CONFIG_DIR.get(f"{x.parent.name}/{x.name}", []),
+        ),
         patch("pathlib.Path.stat", return_value=MagicMock(st_size=123)),
         patch("pathlib.Path.is_file", lambda x: x not in CONFIG_DIR_DIRS),
         patch("pathlib.Path.is_dir", lambda x: x in CONFIG_DIR_DIRS),
diff --git a/tests/components/backup/test_manager.py b/tests/components/backup/test_manager.py
index fef4b84ac61..f518d7c59bc 100644
--- a/tests/components/backup/test_manager.py
+++ b/tests/components/backup/test_manager.py
@@ -68,10 +68,15 @@ from tests.typing import ClientSessionGenerator, WebSocketGenerator
 _EXPECTED_FILES = [
     "test.txt",
     ".storage",
+    "another_subdir",
+    "another_subdir/backups",
+    "another_subdir/backups/not_backup",
+    "another_subdir/tts",
     "backups",
     "backups/not_backup",
     "tmp_backups",
     "tmp_backups/not_backup",
+    "tts",
 ]
 _EXPECTED_FILES_WITH_DATABASE = {
     True: [*_EXPECTED_FILES, "home-assistant_v2.db"],