diff --git a/homeassistant/components/profiler/__init__.py b/homeassistant/components/profiler/__init__.py
index 8b0252e7fa7..8c5c206ae9f 100644
--- a/homeassistant/components/profiler/__init__.py
+++ b/homeassistant/components/profiler/__init__.py
@@ -402,11 +402,6 @@ async def _async_generate_memory_profile(hass: HomeAssistant, call: ServiceCall)
     # Imports deferred to avoid loading modules
     # in memory since usually only one part of this
     # integration is used at a time
-    if sys.version_info >= (3, 12):
-        raise HomeAssistantError(
-            "Memory profiling is not supported on Python 3.12. Please use Python 3.11."
-        )
-
     from guppy import hpy  # pylint: disable=import-outside-toplevel
 
     start_time = int(time.time() * 1000000)
diff --git a/homeassistant/components/profiler/manifest.json b/homeassistant/components/profiler/manifest.json
index eeb0a182ee3..d96f76d25a4 100644
--- a/homeassistant/components/profiler/manifest.json
+++ b/homeassistant/components/profiler/manifest.json
@@ -5,9 +5,5 @@
   "config_flow": true,
   "documentation": "https://www.home-assistant.io/integrations/profiler",
   "quality_scale": "internal",
-  "requirements": [
-    "pyprof2calltree==1.4.5",
-    "guppy3==3.1.3;python_version<'3.12'",
-    "objgraph==3.5.0"
-  ]
+  "requirements": ["pyprof2calltree==1.4.5", "guppy3==3.1.4", "objgraph==3.5.0"]
 }
diff --git a/requirements_all.txt b/requirements_all.txt
index ab134d1a1a4..b2dbedbce46 100644
--- a/requirements_all.txt
+++ b/requirements_all.txt
@@ -948,7 +948,7 @@ gspread==5.5.0
 gstreamer-player==1.1.2
 
 # homeassistant.components.profiler
-guppy3==3.1.3;python_version<'3.12'
+guppy3==3.1.4
 
 # homeassistant.components.iaqualink
 h2==4.1.0
diff --git a/requirements_test_all.txt b/requirements_test_all.txt
index 6aee7925fa3..6ded0033ce7 100644
--- a/requirements_test_all.txt
+++ b/requirements_test_all.txt
@@ -749,7 +749,7 @@ growattServer==1.3.0
 gspread==5.5.0
 
 # homeassistant.components.profiler
-guppy3==3.1.3;python_version<'3.12'
+guppy3==3.1.4
 
 # homeassistant.components.iaqualink
 h2==4.1.0
diff --git a/tests/components/profiler/test_init.py b/tests/components/profiler/test_init.py
index 01f4c4ff510..7c2aeb2a29a 100644
--- a/tests/components/profiler/test_init.py
+++ b/tests/components/profiler/test_init.py
@@ -3,7 +3,6 @@ from datetime import timedelta
 from functools import lru_cache
 import os
 from pathlib import Path
-import sys
 from unittest.mock import patch
 
 from lru import LRU  # pylint: disable=no-name-in-module
@@ -64,9 +63,6 @@ async def test_basic_usage(hass: HomeAssistant, tmp_path: Path) -> None:
     await hass.async_block_till_done()
 
 
-@pytest.mark.skipif(
-    sys.version_info >= (3, 12), reason="not yet available on Python 3.12"
-)
 async def test_memory_usage(hass: HomeAssistant, tmp_path: Path) -> None:
     """Test we can setup and the service is registered."""
     test_dir = tmp_path / "profiles"
@@ -98,24 +94,6 @@ async def test_memory_usage(hass: HomeAssistant, tmp_path: Path) -> None:
     await hass.async_block_till_done()
 
 
-@pytest.mark.skipif(sys.version_info < (3, 12), reason="still works on python 3.11")
-async def test_memory_usage_py312(hass: HomeAssistant, tmp_path: Path) -> None:
-    """Test raise an error on python3.11."""
-    entry = MockConfigEntry(domain=DOMAIN)
-    entry.add_to_hass(hass)
-
-    assert await hass.config_entries.async_setup(entry.entry_id)
-    await hass.async_block_till_done()
-    assert hass.services.has_service(DOMAIN, SERVICE_MEMORY)
-    with pytest.raises(
-        HomeAssistantError,
-        match="Memory profiling is not supported on Python 3.12. Please use Python 3.11.",
-    ):
-        await hass.services.async_call(
-            DOMAIN, SERVICE_MEMORY, {CONF_SECONDS: 0.000001}, blocking=True
-        )
-
-
 async def test_object_growth_logging(
     hass: HomeAssistant, caplog: pytest.LogCaptureFixture
 ) -> None: