diff --git a/homeassistant/components/honeywell/climate.py b/homeassistant/components/honeywell/climate.py
index d12d90a02c3..c285ab83bd1 100644
--- a/homeassistant/components/honeywell/climate.py
+++ b/homeassistant/components/honeywell/climate.py
@@ -28,6 +28,7 @@ from homeassistant.config_entries import ConfigEntry
 from homeassistant.const import ATTR_TEMPERATURE, UnitOfTemperature
 from homeassistant.core import HomeAssistant
 from homeassistant.exceptions import HomeAssistantError
+from homeassistant.helpers import device_registry as dr
 from homeassistant.helpers.device_registry import DeviceInfo
 from homeassistant.helpers.entity_platform import AddEntitiesCallback
 
@@ -97,6 +98,37 @@ async def async_setup_entry(
             for device in data.devices.values()
         ]
     )
+    remove_stale_devices(hass, entry, data.devices)
+
+
+def remove_stale_devices(
+    hass: HomeAssistant,
+    config_entry: ConfigEntry,
+    devices: dict[str, SomeComfortDevice],
+) -> None:
+    """Remove stale devices from device registry."""
+    device_registry = dr.async_get(hass)
+    device_entries = dr.async_entries_for_config_entry(
+        device_registry, config_entry.entry_id
+    )
+    all_device_ids: list = []
+    for device in devices.values():
+        all_device_ids.append(device.deviceid)
+
+    for device_entry in device_entries:
+        device_id: str | None = None
+
+        for identifier in device_entry.identifiers:
+            device_id = identifier[1]
+            break
+
+        if device_id is None or device_id not in all_device_ids:
+            # If device_id is None an invalid device entry was found for this config entry.
+            # If the device_id is not in existing device ids it's a stale device entry.
+            # Remove config entry from this device entry in either case.
+            device_registry.async_update_device(
+                device_entry.id, remove_config_entry_id=config_entry.entry_id
+            )
 
 
 class HoneywellUSThermostat(ClimateEntity):
diff --git a/tests/components/honeywell/test_init.py b/tests/components/honeywell/test_init.py
index f7629fa958e..e5afe311295 100644
--- a/tests/components/honeywell/test_init.py
+++ b/tests/components/honeywell/test_init.py
@@ -12,6 +12,7 @@ from homeassistant.components.honeywell.const import (
 from homeassistant.config_entries import ConfigEntryState
 from homeassistant.const import CONF_PASSWORD, CONF_USERNAME
 from homeassistant.core import HomeAssistant
+from homeassistant.helpers import device_registry as dr
 
 from . import init_integration
 
@@ -33,7 +34,10 @@ async def test_setup_entry(hass: HomeAssistant, config_entry: MockConfigEntry) -
 
 
 async def test_setup_multiple_thermostats(
-    hass: HomeAssistant, config_entry: MockConfigEntry, location, another_device
+    hass: HomeAssistant,
+    config_entry: MockConfigEntry,
+    location: MagicMock,
+    another_device: MagicMock,
 ) -> None:
     """Test that the config form is shown."""
     location.devices_by_id[another_device.deviceid] = another_device
@@ -50,8 +54,8 @@ async def test_setup_multiple_thermostats_with_same_deviceid(
     hass: HomeAssistant,
     caplog: pytest.LogCaptureFixture,
     config_entry: MockConfigEntry,
-    device,
-    client,
+    device: MagicMock,
+    client: MagicMock,
 ) -> None:
     """Test Honeywell TCC API returning duplicate device IDs."""
     mock_location2 = create_autospec(aiosomecomfort.Location, instance=True)
@@ -115,3 +119,48 @@ async def test_no_devices(
     client.locations_by_id = {}
     await init_integration(hass, config_entry)
     assert config_entry.state is ConfigEntryState.SETUP_ERROR
+
+
+async def test_remove_stale_device(
+    hass: HomeAssistant,
+    config_entry: MockConfigEntry,
+    location: MagicMock,
+    another_device: MagicMock,
+    client: MagicMock,
+) -> None:
+    """Test that the stale device is removed."""
+    location.devices_by_id[another_device.deviceid] = another_device
+    config_entry.add_to_hass(hass)
+    await hass.config_entries.async_setup(config_entry.entry_id)
+    await hass.async_block_till_done()
+    assert config_entry.state is ConfigEntryState.LOADED
+    assert (
+        hass.states.async_entity_ids_count() == 6
+    )  # 2 climate entities; 4 sensor entities
+
+    device_registry = dr.async_get(hass)
+    device_entry = dr.async_entries_for_config_entry(
+        device_registry, config_entry.entry_id
+    )
+    assert len(device_entry) == 2
+    assert any((DOMAIN, 1234567) in device.identifiers for device in device_entry)
+    assert any((DOMAIN, 7654321) in device.identifiers for device in device_entry)
+
+    assert await config_entry.async_unload(hass)
+    await hass.async_block_till_done()
+    assert config_entry.state == ConfigEntryState.NOT_LOADED
+
+    del location.devices_by_id[another_device.deviceid]
+
+    await hass.config_entries.async_setup(config_entry.entry_id)
+    await hass.async_block_till_done()
+    assert config_entry.state is ConfigEntryState.LOADED
+    assert (
+        hass.states.async_entity_ids_count() == 3
+    )  # 1 climate entities; 2 sensor entities
+
+    device_entry = dr.async_entries_for_config_entry(
+        device_registry, config_entry.entry_id
+    )
+    assert len(device_entry) == 1
+    assert any((DOMAIN, 1234567) in device.identifiers for device in device_entry)