diff --git a/homeassistant/components/s3/__init__.py b/homeassistant/components/s3/__init__.py
index 95e5e7d738c..ea6b8e244b1 100644
--- a/homeassistant/components/s3/__init__.py
+++ b/homeassistant/components/s3/__init__.py
@@ -7,6 +7,7 @@ from typing import cast
 
 from aiobotocore.client import AioBaseClient as S3Client
 from aiobotocore.session import AioSession
+from botocore.config import Config
 from botocore.exceptions import ClientError, ConnectionError, ParamValidationError
 
 from homeassistant.config_entries import ConfigEntry
@@ -32,6 +33,11 @@ async def async_setup_entry(hass: HomeAssistant, entry: S3ConfigEntry) -> bool:
     """Set up S3 from a config entry."""
 
     data = cast(dict, entry.data)
+    # due to https://github.com/home-assistant/core/issues/143995
+    config = Config(
+        request_checksum_calculation="when_required",
+        response_checksum_validation="when_required",
+    )
     try:
         session = AioSession()
         # pylint: disable-next=unnecessary-dunder-call
@@ -40,6 +46,7 @@ async def async_setup_entry(hass: HomeAssistant, entry: S3ConfigEntry) -> bool:
             endpoint_url=data.get(CONF_ENDPOINT_URL),
             aws_secret_access_key=data[CONF_SECRET_ACCESS_KEY],
             aws_access_key_id=data[CONF_ACCESS_KEY_ID],
+            config=config,
         ).__aenter__()
         await client.head_bucket(Bucket=data[CONF_BUCKET])
     except ClientError as err:
diff --git a/tests/components/s3/test_init.py b/tests/components/s3/test_init.py
index afa11f5cf72..8255bbd0c66 100644
--- a/tests/components/s3/test_init.py
+++ b/tests/components/s3/test_init.py
@@ -2,6 +2,7 @@
 
 from unittest.mock import AsyncMock, patch
 
+from botocore.config import Config
 from botocore.exceptions import (
     ClientError,
     EndpointConnectionError,
@@ -73,3 +74,19 @@ async def test_setup_entry_head_bucket_error(
     )
     await setup_integration(hass, mock_config_entry)
     assert mock_config_entry.state is ConfigEntryState.SETUP_ERROR
+
+
+async def test_checksum_settings_present(
+    hass: HomeAssistant, mock_config_entry: MockConfigEntry
+) -> None:
+    """Test that checksum validation is set to be compatible with third-party S3 providers."""
+    # due to https://github.com/home-assistant/core/issues/143995
+    with patch(
+        "homeassistant.components.s3.AioSession.create_client"
+    ) as mock_create_client:
+        await setup_integration(hass, mock_config_entry)
+
+        config_arg = mock_create_client.call_args[1]["config"]
+        assert isinstance(config_arg, Config)
+        assert config_arg.request_checksum_calculation == "when_required"
+        assert config_arg.response_checksum_validation == "when_required"