diff --git a/homeassistant/components/usb/__init__.py b/homeassistant/components/usb/__init__.py
index 3a05da085d8..c6a18a27b42 100644
--- a/homeassistant/components/usb/__init__.py
+++ b/homeassistant/components/usb/__init__.py
@@ -205,11 +205,17 @@ class USBDiscovery:
         """Set up USB Discovery."""
         await self._async_start_monitor()
         self.hass.bus.async_listen_once(EVENT_HOMEASSISTANT_STARTED, self.async_start)
+        self.hass.bus.async_listen_once(EVENT_HOMEASSISTANT_STOP, self.async_stop)
 
     async def async_start(self, event: Event) -> None:
         """Start USB Discovery and run a manual scan."""
         await self._async_scan_serial()
 
+    async def async_stop(self, event: Event) -> None:
+        """Stop USB Discovery."""
+        if self._request_debouncer:
+            await self._request_debouncer.async_shutdown()
+
     async def _async_start_monitor(self) -> None:
         """Start monitoring hardware with pyudev."""
         if not sys.platform.startswith("linux"):