diff --git a/homeassistant/package_constraints.txt b/homeassistant/package_constraints.txt
index ed9466073dd..7cd0a56c337 100644
--- a/homeassistant/package_constraints.txt
+++ b/homeassistant/package_constraints.txt
@@ -29,7 +29,7 @@ cached-ipaddress==0.10.0
 certifi>=2021.5.30
 ciso8601==2.3.2
 cronsim==2.6
-cryptography==44.0.1
+cryptography==45.0.1
 dbus-fast==2.43.0
 fnv-hash-fast==1.5.0
 go2rtc-client==0.1.2
@@ -55,7 +55,7 @@ psutil-home-assistant==0.0.1
 PyJWT==2.10.1
 pymicro-vad==1.0.1
 PyNaCl==1.5.0
-pyOpenSSL==25.0.0
+pyOpenSSL==25.1.0
 pyserial==3.5
 pyspeex-noise==1.0.2
 python-slugify==8.0.4
@@ -143,10 +143,6 @@ pubnub!=6.4.0
 # https://github.com/dahlia/iso4217/issues/16
 iso4217!=1.10.20220401
 
-# pyOpenSSL 24.0.0 or later required to avoid import errors when
-# cryptography 42.0.0 is installed with botocore
-pyOpenSSL>=24.0.0
-
 # protobuf must be in package constraints for the wheel
 # builder to build binary wheels
 protobuf==5.29.2
diff --git a/pyproject.toml b/pyproject.toml
index 68954726b56..bab4f92bc23 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -82,10 +82,10 @@ dependencies = [
   "numpy==2.2.2",
   "PyJWT==2.10.1",
   # PyJWT has loose dependency. We want the latest one.
-  "cryptography==44.0.1",
+  "cryptography==45.0.1",
   "Pillow==11.2.1",
   "propcache==0.3.1",
-  "pyOpenSSL==25.0.0",
+  "pyOpenSSL==25.1.0",
   "orjson==3.10.18",
   "packaging>=23.1",
   "psutil-home-assistant==0.0.1",
diff --git a/requirements.txt b/requirements.txt
index 25f977d455f..a4ab40f2538 100644
--- a/requirements.txt
+++ b/requirements.txt
@@ -34,10 +34,10 @@ lru-dict==1.3.0
 mutagen==1.47.0
 numpy==2.2.2
 PyJWT==2.10.1
-cryptography==44.0.1
+cryptography==45.0.1
 Pillow==11.2.1
 propcache==0.3.1
-pyOpenSSL==25.0.0
+pyOpenSSL==25.1.0
 orjson==3.10.18
 packaging>=23.1
 psutil-home-assistant==0.0.1
diff --git a/script/gen_requirements_all.py b/script/gen_requirements_all.py
index 307a9c42d53..f2e423536e8 100755
--- a/script/gen_requirements_all.py
+++ b/script/gen_requirements_all.py
@@ -172,10 +172,6 @@ pubnub!=6.4.0
 # https://github.com/dahlia/iso4217/issues/16
 iso4217!=1.10.20220401
 
-# pyOpenSSL 24.0.0 or later required to avoid import errors when
-# cryptography 42.0.0 is installed with botocore
-pyOpenSSL>=24.0.0
-
 # protobuf must be in package constraints for the wheel
 # builder to build binary wheels
 protobuf==5.29.2