diff --git a/homeassistant/components/onewire/config_flow.py b/homeassistant/components/onewire/config_flow.py
index 9ab8dc32f68..c5d4bb065e0 100644
--- a/homeassistant/components/onewire/config_flow.py
+++ b/homeassistant/components/onewire/config_flow.py
@@ -8,6 +8,7 @@ from typing import Any
 from pyownet import protocol
 import voluptuous as vol
 
+from homeassistant.components import zeroconf
 from homeassistant.config_entries import ConfigFlow, ConfigFlowResult, OptionsFlow
 from homeassistant.const import CONF_HOST, CONF_PORT
 from homeassistant.core import HomeAssistant, callback
@@ -115,6 +116,19 @@ class OneWireFlowHandler(ConfigFlow, domain=DOMAIN):
         }
         return await self.async_step_discovery_confirm()
 
+    async def async_step_zeroconf(
+        self, discovery_info: zeroconf.ZeroconfServiceInfo
+    ) -> ConfigFlowResult:
+        """Handle zeroconf discovery."""
+        await self._async_handle_discovery_without_unique_id()
+
+        self._discovery_data = {
+            "title": discovery_info.name,
+            CONF_HOST: discovery_info.hostname,
+            CONF_PORT: discovery_info.port,
+        }
+        return await self.async_step_discovery_confirm()
+
     async def async_step_discovery_confirm(
         self, user_input: dict[str, Any] | None = None
     ) -> ConfigFlowResult:
diff --git a/homeassistant/components/onewire/manifest.json b/homeassistant/components/onewire/manifest.json
index 4f3cb5d04ab..844c4c1afb9 100644
--- a/homeassistant/components/onewire/manifest.json
+++ b/homeassistant/components/onewire/manifest.json
@@ -7,5 +7,6 @@
   "integration_type": "hub",
   "iot_class": "local_polling",
   "loggers": ["pyownet"],
-  "requirements": ["pyownet==0.10.0.post1"]
+  "requirements": ["pyownet==0.10.0.post1"],
+  "zeroconf": ["_owserver._tcp.local."]
 }
diff --git a/homeassistant/components/onewire/quality_scale.yaml b/homeassistant/components/onewire/quality_scale.yaml
index b64bfb775ce..d46ed69f0d6 100644
--- a/homeassistant/components/onewire/quality_scale.yaml
+++ b/homeassistant/components/onewire/quality_scale.yaml
@@ -67,9 +67,7 @@ rules:
   entity-disabled-by-default: done
   discovery:
     status: done
-    comment: |
-      hassio discovery implemented, mDNS/zeroconf should also be possible
-      https://owfs.org/index_php_page_avahi-discovery.html (see PR 135295)
+    comment: hassio and mDNS/zeroconf discovery implemented
   stale-devices:
     status: done
     comment: >
diff --git a/homeassistant/generated/zeroconf.py b/homeassistant/generated/zeroconf.py
index 0766e1ce011..203f01e7d68 100644
--- a/homeassistant/generated/zeroconf.py
+++ b/homeassistant/generated/zeroconf.py
@@ -729,6 +729,11 @@ ZEROCONF = {
             "domain": "octoprint",
         },
     ],
+    "_owserver._tcp.local.": [
+        {
+            "domain": "onewire",
+        },
+    ],
     "_plexmediasvr._tcp.local.": [
         {
             "domain": "plex",
diff --git a/tests/components/onewire/test_config_flow.py b/tests/components/onewire/test_config_flow.py
index 09a7cdfcbb0..db551c0a1c3 100644
--- a/tests/components/onewire/test_config_flow.py
+++ b/tests/components/onewire/test_config_flow.py
@@ -1,5 +1,6 @@
 """Tests for 1-Wire config flow."""
 
+from ipaddress import ip_address
 from unittest.mock import AsyncMock, patch
 
 from pyownet import protocol
@@ -11,7 +12,8 @@ from homeassistant.components.onewire.const import (
     INPUT_ENTRY_DEVICE_SELECTION,
     MANUFACTURER_MAXIM,
 )
-from homeassistant.config_entries import SOURCE_HASSIO, SOURCE_USER
+from homeassistant.components.zeroconf import ZeroconfServiceInfo
+from homeassistant.config_entries import SOURCE_HASSIO, SOURCE_USER, SOURCE_ZEROCONF
 from homeassistant.const import CONF_HOST, CONF_PORT
 from homeassistant.core import HomeAssistant
 from homeassistant.data_entry_flow import FlowResultType
@@ -26,6 +28,15 @@ _HASSIO_DISCOVERY = HassioServiceInfo(
     slug="1302b8e0_owserver",
     uuid="e3fa56560d93458b96a594cbcea3017e",
 )
+_ZEROCONF_DISCOVERY = ZeroconfServiceInfo(
+    ip_address=ip_address("5.6.7.8"),
+    ip_addresses=[ip_address("5.6.7.8")],
+    hostname="ubuntu.local.",
+    name="OWFS (1-wire) Server",
+    port=4304,
+    type="_owserver._tcp.local.",
+    properties={},
+)
 
 pytestmark = pytest.mark.usefixtures("mock_setup_entry")
 
@@ -250,6 +261,58 @@ async def test_hassio_duplicate(hass: HomeAssistant) -> None:
     assert result["reason"] == "already_configured"
 
 
+async def test_zeroconf_flow(hass: HomeAssistant) -> None:
+    """Test zeroconf discovery flow."""
+    result = await hass.config_entries.flow.async_init(
+        DOMAIN,
+        context={"source": SOURCE_ZEROCONF},
+        data=_ZEROCONF_DISCOVERY,
+    )
+    assert result["type"] is FlowResultType.FORM
+    assert result["step_id"] == "discovery_confirm"
+    assert not result["errors"]
+
+    # Cannot connect to server => retry
+    with patch(
+        "homeassistant.components.onewire.onewirehub.protocol.proxy",
+        side_effect=protocol.ConnError,
+    ):
+        result = await hass.config_entries.flow.async_configure(
+            result["flow_id"],
+            user_input={},
+        )
+
+    assert result["type"] is FlowResultType.FORM
+    assert result["step_id"] == "discovery_confirm"
+    assert result["errors"] == {"base": "cannot_connect"}
+
+    # Connect OK
+    with patch(
+        "homeassistant.components.onewire.onewirehub.protocol.proxy",
+    ):
+        result = await hass.config_entries.flow.async_configure(
+            result["flow_id"],
+            user_input={},
+        )
+
+    assert result["type"] is FlowResultType.CREATE_ENTRY
+    new_entry = result["result"]
+    assert new_entry.title == "OWFS (1-wire) Server"
+    assert new_entry.data == {CONF_HOST: "ubuntu.local.", CONF_PORT: 4304}
+
+
+@pytest.mark.usefixtures("config_entry")
+async def test_zeroconf_duplicate(hass: HomeAssistant) -> None:
+    """Test zeroconf discovery duplicate flow."""
+    result = await hass.config_entries.flow.async_init(
+        DOMAIN,
+        context={"source": SOURCE_ZEROCONF},
+        data=_ZEROCONF_DISCOVERY,
+    )
+    assert result["type"] is FlowResultType.ABORT
+    assert result["reason"] == "already_configured"
+
+
 @pytest.mark.usefixtures("filled_device_registry")
 async def test_user_options_clear(
     hass: HomeAssistant, config_entry: MockConfigEntry