diff --git a/homeassistant/components/spotify/__init__.py b/homeassistant/components/spotify/__init__.py
index 59ebf1ead55..c057ea240c0 100644
--- a/homeassistant/components/spotify/__init__.py
+++ b/homeassistant/components/spotify/__init__.py
@@ -30,7 +30,11 @@ from homeassistant.helpers.update_coordinator import DataUpdateCoordinator, Upda
 from . import config_flow
 from .browse_media import async_browse_media
 from .const import DOMAIN, LOGGER, SPOTIFY_SCOPES
-from .util import is_spotify_media_type, resolve_spotify_media_type
+from .util import (
+    is_spotify_media_type,
+    resolve_spotify_media_type,
+    spotify_uri_from_media_browser_url,
+)
 
 CONFIG_SCHEMA = vol.Schema(
     {
@@ -50,6 +54,7 @@ PLATFORMS = [Platform.MEDIA_PLAYER]
 __all__ = [
     "async_browse_media",
     "DOMAIN",
+    "spotify_uri_from_media_browser_url",
     "is_spotify_media_type",
     "resolve_spotify_media_type",
 ]
diff --git a/homeassistant/components/spotify/browse_media.py b/homeassistant/components/spotify/browse_media.py
index efaa07b3172..db2379a57fc 100644
--- a/homeassistant/components/spotify/browse_media.py
+++ b/homeassistant/components/spotify/browse_media.py
@@ -6,11 +6,13 @@ import logging
 from typing import Any
 
 from spotipy import Spotify
+import yarl
 
 from homeassistant.backports.enum import StrEnum
 from homeassistant.components.media_player import BrowseError, BrowseMedia
 from homeassistant.components.media_player.const import (
     MEDIA_CLASS_ALBUM,
+    MEDIA_CLASS_APP,
     MEDIA_CLASS_ARTIST,
     MEDIA_CLASS_DIRECTORY,
     MEDIA_CLASS_EPISODE,
@@ -137,15 +139,53 @@ class UnknownMediaType(BrowseError):
 
 async def async_browse_media(
     hass: HomeAssistant,
-    media_content_type: str,
-    media_content_id: str,
+    media_content_type: str | None,
+    media_content_id: str | None,
     *,
     can_play_artist: bool = True,
 ) -> BrowseMedia:
     """Browse Spotify media."""
-    if not (info := next(iter(hass.data[DOMAIN].values()), None)):
-        raise BrowseError("No Spotify accounts available")
-    return await async_browse_media_internal(
+    parsed_url = None
+    info = None
+
+    # Check if caller is requesting the root nodes
+    if media_content_type is None and media_content_id is None:
+        children = []
+        for config_entry_id, info in hass.data[DOMAIN].items():
+            config_entry = hass.config_entries.async_get_entry(config_entry_id)
+            assert config_entry is not None
+            children.append(
+                BrowseMedia(
+                    title=config_entry.title,
+                    media_class=MEDIA_CLASS_APP,
+                    media_content_id=f"{MEDIA_PLAYER_PREFIX}{config_entry_id}",
+                    media_content_type=f"{MEDIA_PLAYER_PREFIX}library",
+                    thumbnail="https://brands.home-assistant.io/_/spotify/logo.png",
+                    can_play=False,
+                    can_expand=True,
+                )
+            )
+        return BrowseMedia(
+            title="Spotify",
+            media_class=MEDIA_CLASS_APP,
+            media_content_id=MEDIA_PLAYER_PREFIX,
+            media_content_type="spotify",
+            thumbnail="https://brands.home-assistant.io/_/spotify/logo.png",
+            can_play=False,
+            can_expand=True,
+            children=children,
+        )
+
+    if media_content_id is None or not media_content_id.startswith(MEDIA_PLAYER_PREFIX):
+        raise BrowseError("Invalid Spotify URL specified")
+
+    # Check for config entry specifier, and extract Spotify URI
+    parsed_url = yarl.URL(media_content_id)
+    if (info := hass.data[DOMAIN].get(parsed_url.host)) is None:
+        raise BrowseError("Invalid Spotify account specified")
+    media_content_id = parsed_url.name
+
+    result = await async_browse_media_internal(
         hass,
         info.client,
         info.session,
@@ -155,6 +195,13 @@ async def async_browse_media(
         can_play_artist=can_play_artist,
     )
 
+    # Build new URLs with config entry specifyers
+    result.media_content_id = str(parsed_url.with_name(result.media_content_id))
+    if result.children:
+        for child in result.children:
+            child.media_content_id = str(parsed_url.with_name(child.media_content_id))
+    return result
+
 
 async def async_browse_media_internal(
     hass: HomeAssistant,
diff --git a/homeassistant/components/spotify/util.py b/homeassistant/components/spotify/util.py
index cdb8e933523..7f7f682fb9e 100644
--- a/homeassistant/components/spotify/util.py
+++ b/homeassistant/components/spotify/util.py
@@ -3,6 +3,8 @@ from __future__ import annotations
 
 from typing import Any
 
+import yarl
+
 from .const import MEDIA_PLAYER_PREFIX
 
 
@@ -22,3 +24,11 @@ def fetch_image_url(item: dict[str, Any], key="images") -> str | None:
         return item.get(key, [])[0].get("url")
     except IndexError:
         return None
+
+
+def spotify_uri_from_media_browser_url(media_content_id: str) -> str:
+    """Extract spotify URI from media browser URL."""
+    if media_content_id and media_content_id.startswith(MEDIA_PLAYER_PREFIX):
+        parsed_url = yarl.URL(media_content_id)
+        media_content_id = parsed_url.name
+    return media_content_id